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KMID : 1237720230560040518
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Anatomy & Cell Biology
2023 Volume.56 No. 4 p.518 ~ p.525
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The correlation of Septin4 gene expression with sperm quality, DNA damage, and oxidative stress level in infertile patients
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Rahil Jannatifar
Hamid Piroozmanesh
Fahimeh Naghi Jalalabadi
Hamid Reza Momeni
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Abstract
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Septin4 belong to a family of polymerizing GTP-binding proteins that are required for many cellular functions,such as membrane compartmentalization, vesicular trafficking, mitosis, and cytoskeletal remodeling. Since, Septin4 isexpressed specifically in the testis, we aimed to determine the association between Septin4 gene expression with spermquality, DNA damage, and stress oxidative level in infertile patients. The present study included 60 semen samples thatgrouped into three groups: normozoospermia (n=20), asthenozoospermia (n=20), astheno-teratozoospermia (n=20). Initially,semen parameters were analyzed by using the World Health Organization protocol. The mRNA expression of Septin4 insperm was examined using reverse transcription-polymerase chain reaction. Oxidative stress markers, i.e., total antioxidantcapacity, superoxide dismutase, catalase, glutathione peroxidase, and malondialdehyde, were determined by ELISA kit. Thecurrent study showed a statistically significant highly positive correlation in Septin4 gene expression with sperm motility,normal morphology, viability, capacity, and sperm mitochondrial membrane potential (MMP). However, it showed significantnegative correlation with sperm DNA fragmentation. Septin4 had a significant correlation with stress oxidative factor andantioxidant enzyme levels. In conclusion, Septin4 gene expression provides clinical useful information for the diagnosis ofmale infertility. It might be a marker for discrimination between fertile and infertile patients. The current study showeda statistically significant highly positive correlation in Septin4 gene expression with sperm motility, normal morphology,viability, capacity, and sperm MMP. However, it shows significant negative correlation with sperm DNA fragmentation.
Septin4 had a significant correlation with stress oxidative factor and antioxidant enzyme levels.
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KEYWORD
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Septin4, Spermatozoa, DNA damage, Oxidative stress
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